sub:provenance { beldoc:dce:description "Approximately 2000 hand curated statements drawn from 57 PubMeds." ; dce:rights "Copyright (c) 2011-2012, Selventa. All Rights Reserved." ; dce:title "BEL Framework Small Corpus Document" ; dct:license "Creative Commons Attribution-Non-Commercial-ShareAlike 3.0 Unported License" ; pav:authoredBysub:_6 ; pav:version "20131211" . sub:_5prov:value """VEGF induced tyrosine phosphorylation of the regulatory p85 subunit of PI 3-kinase, evidenced by immunoblotting of anti-p85 immunoprecipitates with an anti-pY mAb (Fig. 5b). Moreover, immunoblotting of anti-pY immunoprecipitates with specific p85 mAb showed an increased association of p85 with tyrosine-phosphorylated proteins, which was maximal after 5 min of VEGF treatment (Fig. 5c). To determine whether VEGF-induced p85 phosphorylation regulates p110, the catalytic counterpart of PI 3-kinase, we performed in vitrokinase assays using PI 4,5-bisphosphate as a substrate. As shown in Fig. 5d, VEGF induced peak activation (6-fold) of PI 3-kinase activity at 5 min.""" ; prov:wasQuotedFrompubmed:11751905 . sub:_6rdfs:comment "support@belframework.org" ; rdfs:label "Selventa" . sub:assertionprov:hadPrimarySourcepubmed:11751905 ; prov:wasDerivedFrombeldoc: , sub:_5 . }