http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#Head http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#provenance http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#pubinfo http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_1 http://purl.obolibrary.org/obo/RO_0002204 http://resource.belframework.org/belframework/1.0/namespace/selventa-named-human-protein-families/VEGF%20Family http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_2 http://semanticscience.org/resource/SIO_000139 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_3 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_2 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://amigo.geneontology.org/amigo/term/GO:0016301 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_3 http://purl.obolibrary.org/obo/RO_0002204 http://resource.belframework.org/belframework/1.0/namespace/selventa-named-human-protein-families/AKT%20Family http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_3 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_2 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_1 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_4 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D002478 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D014471 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://purl.obolibrary.org/obo/BFO_0000066 http://purl.bioontology.org/ontology/MSH/D042783 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://purl.obolibrary.org/obo/BFO_0000066 http://www.lgcstandards-atcc.org/Products/All/CRL-1730.aspx http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://semanticscience.org/resource/SIO_000255 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_6 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_4 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/decreases http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://resource.belframework.org/belframework/1.0/namespace/selventa-legacy-chemical-names/Smoke%20extract%2C%20cigarette http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_5 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_6 http://purl.org/dc/terms/subject TextLocation http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_6 http://www.w3.org/1999/02/22-rdf-syntax-ns#value Results http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.w3.org/2000/01/rdf-schema#label a(SCHEM:"Smoke extract, cigarette") -| (p(PFH:"VEGF Family") -> kin(p(PFH:"AKT Family"))) http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.w3.org/2000/01/rdf-schema#label p(PFH:"VEGF Family") -> kin(p(PFH:"AKT Family")) http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#provenance http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 2000 hand curated statements drawn from 57 PubMeds. http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All Rights Reserved. http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Small Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://purl.org/dc/terms/license Creative Commons Attribution-Non-Commercial-ShareAlike 3.0 Unported License http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_8 http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://purl.org/pav/version 1.6 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_7 http://www.w3.org/ns/prov#value Because NO is an essential mediator of endothelial cell migration and VEGF-induced angiogenesis, we investigated the effect of CSE exposure on VEGF-dependent Akt/eNOS/NO pathway. VEGF-stimulated HUVECs exposed to CSE show a dose-dependent inhibition of phosphorylated Akt and eNOS (Fig. 4A). Importantly, the activation of Akt and eNOS by VEGF can be rescued following treatment with the antioxidants NAC and vitamin C (Fig. 4B). Similarly, we found that the induction of NO by VEGF in endothelial cell cultures was severely compromised in the presence of CSE, but that NO levels could be normalized with antioxidants (Fig. 4C). Globally, these data suggest that ROS are involved in the impairment of VEGF-induced Akt/eNOS/NO pathway by CSE. http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_7 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/16806264 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_8 http://www.w3.org/2000/01/rdf-schema#comment support@belframework.org http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_8 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/16806264 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/small_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#_7 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330#pubinfo http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://purl.org/dc/terms/created 2014-07-03T14:29:31.651+02:00 http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAVosfAgf0wTfFeJ4juR2VcwDa2BZaPwBDKy4cHMta330 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234