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All Rights Reserved. http://resource.belframework.org/belframework/20131211/knowledge/small_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Small Corpus Document http://resource.belframework.org/belframework/20131211/knowledge/small_corpus.bel http://purl.org/dc/terms/license Creative Commons Attribution-Non-Commercial-ShareAlike 3.0 Unported License http://resource.belframework.org/belframework/20131211/knowledge/small_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#_7 http://resource.belframework.org/belframework/20131211/knowledge/small_corpus.bel http://purl.org/pav/version 20131211 http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#_6 http://www.w3.org/ns/prov#value We next examined whether mutations of this MEF2 site affects MEF2 activity on the PGC-1α promoter. The mutated 2-kb promoter (called ΔMEF2; see Fig. 3A) is no longer able to mediate MEF2C or MEF2D induction alone, when activated by CnA or coactivated by PGC-1α, suggesting that this site is responsible for the MEF2 action (Fig. 3B). Moreover, electrophoretic mobility-shift assays revealed binding of MEF2C to this MEF-binding site but not the mutated ΔMEF site in the PGC-1α promoter (Fig. 3C). The identity of the MEF2C–DNA complex was confirmed by using an anti-MEF2 antibody that leads to a supershift of the DNA–protein complex. http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#_6 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12764228 http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#_7 http://www.w3.org/2000/01/rdf-schema#comment support@belframework.org http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#_7 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12764228 http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/20131211/knowledge/small_corpus.bel http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#_6 http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho#pubinfo http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho http://purl.org/dc/terms/created 2014-07-03T14:31:18.167+02:00 http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RA1FtKvkgumU8hWoq27bs1espLPZgdMtBphKCgxpi8Tho http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234